Genetic loci associated with <i>Fusarium solani </i>tolerance in soybean

摘要

The invention relates to methods and compositions for identifying soybean plants that are tolerant, have improved tolerance or are susceptible to Fusarium solani infection (the causative agent of sudden death syndrome or SDS). The methods use molecular genetic markers to identify, select and/or construct disease-tolerant plants or identify and counter-select disease-susceptible plants. Soybean plants that display tolerance or improved tolerance to Fusarium solani infection that are generated by the methods of the invention are also a feature of the invention.

主权项

1. A method of identifying a first soybean plant or germplasm that displays tolerance or improved tolerance to Fusarium solani infection, the method comprising isolating nucleic acids from a genome of the first soybean plant or germplasm, detecting in the first soybean plant or germplasm at least one allele of one or more marker locus that is associated with the tolerance or improved tolerance wherein at least one allele is detected using a composition comprising a detectable label, and selecting said first soybean plant or germplasm, or selecting a progeny of said first soybean plant or germplasm wherein the plant, germplasm or progeny comprises at least one allele associated with tolerance or improved tolerance to Fusarium, and wherein the one or more marker locus is selected from the group consisting of:
(a) SATT166, SATT448, SATT678, SAT 099 S60375-TB, SATT006, SATT229 SATT373, SAT 340, Sct—010, SATT076, SAT113, SATT678; SATT527, SATT561, and A132 2; and (b) a marker locus closely linked to a marker locus of (a).