| 发明名称 | Method for producing a polypeptide using a cell that overexpresses a bicarbonate transporter | ||
| 摘要 | The present invention provides a method capable of producing a protein efficiently. A method of producing a polypeptide, comprising culturing a cell which strongly expresses a bicarbonate transporter and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce said polypeptide. | ||
| 申请公布号 | US9068212(B2) | 申请公布日期 | 2015.06.30 |
| 申请号 | US200812734283 | 申请日期 | 2008.10.23 |
| 申请人 | Chugai Seiyaku Kabushiki Kaisha | 发明人 | Tabuchi Hisahiro;Tainaka Satoshi;Sugiyama Tomoya |
| 分类号 | C12P21/02;C12N5/00;C12N5/07;C12N5/16;C07K16/28;C07K16/30 | 主分类号 | C12P21/02 |
| 代理机构 | Foley & Lardner LLP | 代理人 | Foley & Lardner LLP |
| 主权项 | 1. A method of producing an antibody comprising culturing an isolated mammalian cell which is transfected with a heterologous DNA encoding a bicarbonate transporter and is transfected with a heterologous DNA encoding a desired antibody and allowing the cell to produce said antibody, wherein the bicarbonate transporter exchanges Cl− outside of a plasma membrane for HCO3− inside a plasma membrane, and wherein the bicarbonate transporter is anion exchanger 1 (AE1), anion exchanger 2 (AE2), or anion exchanger 3 (AE3). | ||
| 地址 | Tokyo JP | ||