Method and instrumentation for comprehensive multidimensional chromatography separations using a micro flow modulator

摘要

The present invention refers to a modulator, to be used for comprehensive multidimensional chromatography separations, to entrap and release sample solute fractions (entrapped in a capillary loop of fixed or variable volume), deriving from a capillary column with an internal diameter ranging from 0.01 mm to 0.53 mm, onto another capillary column with an internal diameter ranging from 0.01 mm to 0.53 mm. The micro-device has been integrated in a gas chromatographic system, composed of two ovens for the independent temperature control of the two columns; the micro-device is characterized, internally, by a system of channels that enable the controlled splitting of gas flow, entering the second capillary, to generate an optimum gas linear velocity and to release the pressure in excess, with the objective of attaining the maximum separation efficiency in the second column. Furthermore, the system is equipped with a second device, to divide the flow exiting the second column in two different detectors that operate at the same or at different pressures.

主权项

1. A device for chromatography comprising:
a modulator a port that receives effluent from a first chromatographic column; either one or two capillary sample loops located externally with respect to said modulator or either one or two capillary sample loops integrated in said modulator; two independent ports of the modulator that receive a flow from one or more electronic pressure and/or flow control devices located externally with respect to said modulator; three channels either integrated or located externally with respect to said modulator connected to said one or two capillary sample loops and a second column to split with a needle valve the flow exiting an accumulation capillary loop and to release the pressure in excess within the channels connected to said accumulation capillary loop and in the second column when emptying said accumulation capillary loop; a needle valve, either integrated or external to the modulator that regulates the flow exiting said accumulation capillary loop; and a system of integrated communicating channels that connect different ports, wherein said communicating channels are located internally to the modulator and are connected to one another via said accumulation capillary sample loop and said communicating channels are connected to said second column and to a capillary column.