Lateral flow assays with time delayed components

摘要

A lateral flow device may include one or more enhancement elements, where the enhancement elements bind to the analyte sandwich to increase a detection signal in the test zone. In preferred embodiments, some or all of the enhancement elements are encapsulated.

主权项

1. A lateral flow device for detecting an analyte comprising:
a test strip comprising a sample application zone and a test zone; a first conjugate comprising a first binding partner for the analyte and a label; a second binding partner for the analyte capable of being immobilized in the test zone; and at least one encapsulated enhancement element; wherein the analyte, the conjugate, and the second binding partner form a sandwich which is immobilized in the test zone when the analyte is present; and wherein the encapsulated enhancement element binds to the sandwich after being released from encapsulation to increase a detection signal in the test zone.