Compensator for multiple surface imaging

摘要

A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

主权项

1. A system, comprising:
a flow cell comprising a first interior surface and a second interior surface in a generally mutually facing relation, wherein a plurality of nucleic acid sites is distributed on the first interior surface and on the second interior surface, and wherein the first interior surface and the second interior surface are separated by a fluid passage through which a fluorescent reagent flows to add fluorescent tags to the nucleic acid sites; and a detection system configured to detect the fluorescent tags at a resolution to distinguish the nucleic acid sites on the first interior surface, and to detect fluorescent tags at a resolution to distinguish the nucleic acid sites on the second interior surface.