Methods and compositions for a soybean in-planta transient expression system

摘要

This invention provides methods of transiently expressing a nucleotide sequence in one or more cells of a soybean plant, comprising: a) abrading the surface of a soybean plant and/or intact part thereof; and either (b1) immersing the abraded plant and/or abraded intact part thereof in a solution comprising Agrobacterium cells and a surfactant, wherein the Agrobacterium cells comprise the nucleotide sequence to be transiently expressed; (b2) applying a vacuum to the immersed soybean plant and/or intact part thereof of step (b1); and (b3) releasing the vacuum, thereby introducing the solution into the plant and/or intact part thereof; or (c) contacting the abraded plant and/or abraded intact part thereof with a solution comprising Agrobacterium cells and a surfactant, wherein the Agrobacterium cells comprise the nucleotide sequence to be transiently expressed, whereby the nucleotide sequence is transiently expressed in one or more cells of the plant.

主权项

1. A method of transiently expressing one or more nucleotide sequences in one or more cells of an intact whole soybean plant, comprising:
a) contacting the surface of an intact soybean leaf of a whole soybean plant with an abrasive; b) contacting the abraded soybean leaf and/or the whole soybean plant comprising the leaf with a solution comprising bacterial cells competent for nucleic acid transfer and a surfactant, wherein the bacterial cells comprise the one or more nucleotide sequence(s) to be transiently expressed, and wherein said bacterial cells are Agrobacterium, Rhizobuim, Mesorhizobium, or Sinorhizobium cells; c) applying a vacuum to the contacted soybean leaf of step (b) and/or the whole soybean plant comprising said leaf of step (b); and d) releasing the vacuum, thereby introducing the solution into the abraded soybean leaf,whereby the one or more nucleotide sequences are transiently expressed in one or more cells of the soybean plant.