Process for making improved chromatography media and method of use

摘要

The present invention further is directed to the process for making a macroporous polymeric media with covalently bonded quaternary ammonium polymer chains. The media can be applied for chromatographic purification, resulting in porous substrates having improved protein binding capacity and resin selectivity, as well as methods relating to making and using the same.

主权项

1. A method of preparing an adsorbent material for chromatography comprising immobilizing a polymer ligand onto a polymeric support,
wherein said polymeric support has reactive groups selected from the group consisting of aldehydes, active esters, activated alcohols, epoxides, carbonates, carbocations, and combinations thereof, wherein the polymeric support is reacted with the polymer ligand comprising a polymer backbone, thereby forming one linkage between the polymer backbone and the polymeric support, each linkage comprises one or more functional groups; wherein the polymer ligand is prepared by polymerizing a monomer mixture comprising at least one vinyl monomer having one or more functional groups in the presence of a dual-functional chain transfer agent, wherein said chain transfer agent comprises a group which allows for the free radical chain to be transferred and a functional group suitable for reaction of the polymeric ligand with the polymeric support.