Modification of enzymatic crosslinkers for controlling properties of crosslinked matrices

摘要

Improved matrix or hydrogel that is formed by enzymatic crosslinking of polymers wherein the crosslinking enzyme molecules have been modified for the purpose of improving the crosslinking density, mechanical properties, or other properties of the matrix, and/or to provide improved control over the rate and/or extent of crosslinking, wherein the enzyme molecules are modified to alter the perceived volume of the enzyme molecules in the crosslinked matrix being formed. Methods of production and of use are also provided.

主权项

1. A cross-linked matrix, comprising a substrate polymer and a modified enzyme molecule, wherein said substrate polymer has been crosslinked by said modified enzyme molecule, wherein both said modified enzyme molecule and said substrate polymer are present in the matrix, said modified enzyme molecule having a modification selected from the group consisting of:
a modification that increases the actual size of said enzyme molecule; or a modification that increases the hydrodynamic volume of said enzyme molecule wherein said modified enzyme has a reduced cross-linking rate in comparison to non-modified enzyme that results in a lesser extent of crosslinking of said substrate polymer in comparison to a matrix formed with non-modified enzyme.