| 摘要 |
<p>as a biosensor for electrochemical detection of activated platelets and platelet-derived microparticles. A process for producing modified electrode which can be used as a biosensor comprising polishing glassy carbon electrode with alumina slurry, pouring graphene oxide nano material solution on each electrode to cover the whole surface, drying the electrode and washing the same with phosphate buffered saline, pouring EDC and NHS on the electrodes to activate the carboxyl groups on graphene oxide, washing excess EDC-NHS with PBS pouring PAC-1 antibody on each electrode storing the electrodes in humid chamber for at least 1 hour subjecting the electrodes to the step of rinsing with PBS and stored for blocking with 2% bovine serum albumin (BSA) solution inside humid chamber, rinsing the BSA blocked electrodes with Milli- Q water and dried at room temperature in desiccators.</p> |
