| 摘要 |
The present invention concerns fluorescence microscopy, and more specifically, a method for detecting fluorescence emitted from a sample labeled with at least four predetermined fluorochromes, wherein the at least four different fluorochromes comprises a first and a second fluorochrome that forms a pair of fluorochromes, the pair of fluorochromes selected as a Cy3 fluorochrome analog forming the first fluorochrome, and a 594 fluorochrome analog forming the second fluorochrome, or the pair of fluorochromes is selected as a 425 fluorochrome analog forming the first fluorochrome and a 488 fluorochrome analog forming the second fluorochrome. Detection of fluorescence from the at least four predetermined fluorochromes are achieved, in accordance with the invention, by selecting an excitation wavelength interval for the second fluorochrome such that excitation of the first fluorochrome is reduced, and by selecting an emission wavelength range of the first fluorochrome so that the perfusion of the light emission from the second fluorochrome is reduced. |
