| 主权项 |
1. A method for converting non-methylated cytosine in a single-stranded DNA to uracil, comprising subjecting the single-stranded DNA to bisulfite reaction in the presence of at least one of compounds shown by the following general formula [1] to [8];
a compound shown by the following general formula [1] wherein R1 to R6 represent each independently a hydrogen atom or an alkyl group having 1 to 6 carbon atoms; n represents an integer of 1 to 3; a compound shown by the following general formula [2] wherein Y represents a carbon atom, an oxygen atom or a nitrogen atom; R7 to R12 represent each independently a hydrogen atom or an alkyl group having 1 to 6 carbon atoms; k represents an integer of 0 to 2, but when Y is an oxygen atom, k represents 0, when Y is a nitrogen atom, k represents 1, and when Y is a carbon atom, k represents 2; a compound shown by the following general formula [3] wherein R13 to R15 represent each independently a hydrogen atom or an alkyl group having 1 to 6 carbon atoms; a compound shown by the following general formula [4] wherein R17 and R19 represent each independently a hydrogen atom, an amino group or an alkyl group having 1 to 6 carbon atoms; R16, R18 and R20 represent each independently a hydrogen atom, an amino group, an alkyl group having 1 to 6 carbon atoms or a dialkylamino group having 2 to 6 carbon atoms; and R16 and R17 may form a benzene ring together with a carbon atom adjacent to R16 and a carbon atom adjacent to R17; a compound shown by the following general formula [5] wherein R21 and R22 represent each independently an alkyl group having 1 to 6 carbon atoms; and X2 represents a counter anion; a compound shown by the following general formula [6] wherein R23 represents an alkyl group having 1 to 6 carbon atoms; R24 to R28 represent each independently a hydrogen atom or an alkyl group having 1 to 6 carbon atoms; and X2 represents a counter anion; a compound shown by the following general formula [7] wherein R29 to R34 represent each independently a hydrogen atom or an alkyl group having 1 to 6 carbon atoms; a compound shown by the following general formula [8] wherein Z represents a nitrogen atom or a carbon atom; m represents an integer of 0 or 1, and when Z is a nitrogen atom, m represents 0, and when Z is a carbon atom, m represents 1; R35 to R39 represent each independently a hydrogen atom or an alkyl group having 1 to 6 carbon atoms. |
