Method For Coding Of Multiple PCR Reactions For Assay Recognition

摘要

The present invention provides for methods and compositions that use fluorescent dyes for the identification of reagents and solutions that are used to perform PCR assays.

主权项

1. A method for preparing a plurality of reaction mixtures for performing polymerase chain reaction (PCR) amplification of a plurality of target nucleic acids, comprising:
providing a first mastermix solution comprising at least one substance required for performing PCR amplification of a first target nucleic acid, and further comprising a first fluorescent dye and a second fluorescent dye, wherein said first dye and said second dye are present at a predetermined concentration ratio that is unique for said first reagent solution; providing a second mastermix solution comprising at least one substance required for performing PCR amplification of a second target nucleic acid, and further comprising said first fluorescent dye and said second fluorescent dye, wherein said first dye and said second dye are present at a predetermined concentration ratio different from the concentration ratio for said first mastermix solution and that is unique for said second mastermix solution; adding said first mastermix solution to a first reaction mixture to perform PCR amplification of said first target nucleic acid; and adding said second mastermix solution to a second reaction mixture to perform PCR amplification of said second target nucleic acid.