IN VITRO MEMBRANE PROTEIN MOLECULAR EVOLUTIONARY ENGINEERING TECHNIQUE

摘要

The objective of the present invention is to improve the efficiency of screening/selection of a membrane protein in molecular evolutionary engineering (for example, an enzyme evolutionary method).;The above-described objective is achieved by providing a unilamellar liposome comprising:
(a) a DNA comprising a promoter sequence, a translational initiation sequence, and a sequence encoding a membrane protein;(b) an RNA polymerase;(c) a ribonucleotide; and(d) a cell-free protein synthesis system. In one aspect of the present invention, the membrane protein is a transporter, and the unilamellar liposome further comprises(e) a factor that binds to a ligand transported by the membrane protein.

主权项

1. A unilamellar liposome comprising:
(a) a DNA comprising a promoter sequence, a translational initiation sequence, and a sequence encoding a membrane protein; (b) an RNA polymerase; (c) a ribonucleotide; and (d) a cell-free protein synthesis system, wherein the unilamellar liposome is treated with a nuclease, and the nuclease is selected from the group consisting of a ribonuclease and a deoxyribonuclease.