发明名称 |
IMPROVED RECOMBINANT POLYPEPTIDE PRODUCTION METHODS |
摘要 |
Herein is reported a method for producing a fusion-polypeptide comprising the steps of a) cultivating a mammalian cell comprising a nucleic acid encoding a variant fusion-polypeptide wherein the amino acid sequence of the fusion-polypeptide has been modified by replacing in a pro-fusion-polypeptide the endogenous protease cleavage site between the pro-peptide and the fusion-polypeptide with an exogenous (with respect to the origins of the parts of the fusion-polypeptide) or artificial protease cleavage site, and b) recovering the fusion-polypeptide or fusion-pro-polypeptide from the cell or the cultivation medium and thereby producing the (recombinant) fusion-polypeptide. |
申请公布号 |
CA2929149(A1) |
申请公布日期 |
2015.06.25 |
申请号 |
CA20142929149 |
申请日期 |
2014.12.10 |
申请人 |
F. HOFFMANN-LA ROCHE AG |
发明人 |
KOPETZKI, ERHARD;NIEWOEHNER, JENS;MAIER, PETER |
分类号 |
C12P21/00;C12P21/02 |
主分类号 |
C12P21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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