发明名称 |
STIRRER SYSTEM |
摘要 |
The present invention concerns a stirrer system for animal cell culture consisting of a combination of at least one radially-conveying stirrer element and at least one axially-conveying stirrer element, wherein at least three stirrer elements must be present and the uppermost stirrer element is an axially-conveying stirrer element. The stirrer elements are arranged at a certain distance above one another on a stirrer shaft. A particular embodiment is a multiple stirrer system consisting of two disk stirrers as radially-conveying stirrer elements and an inclined-blade stirrer as an axially-conveying stirrer element wherein the inclined-blade stirrer is arranged above the disk stirrer on the stirrer shaft. The stirrer system according to the invention achieves among others a gentler and better intermixing in the culture of shear-sensitive mammalian cells in cell cultures. |
申请公布号 |
US2015175952(A1) |
申请公布日期 |
2015.06.25 |
申请号 |
US201414554749 |
申请日期 |
2014.11.26 |
申请人 |
Hoffmann-La Roche Inc. |
发明人 |
Alisch Alexander;Jenzsch Marco;Pohlscheidt Michael;Thiele Joerg;Wallerius Claus |
分类号 |
C12M1/06;C07K16/40;C07K16/28;B01F7/16;C12N5/071 |
主分类号 |
C12M1/06 |
代理机构 |
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代理人 |
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主权项 |
1. A method for culturing mammalian cells, the method comprising culturing the mammalian cells in a device comprising a stirrer system and a cultivation vessel, wherein the stirrer system consists of two radially-conveying stirrer elements and one axially-conveying stirrer element arranged above one another on a vertical stirrer shaft, wherein the axially-conveying stirrer element is arranged above the two radially-conveying stirrer elements. |
地址 |
Little Falls NJ US |