| 摘要 |
The disclosure provides methods and kits for isolating miRNAs from biological fluids. Specifically, the method comprising contacting a biological fluid with a surface active agent and an anti-miRNA-binding protein reagent, wherein the surface active agent dissociates biological fluid components and the anti-miRNA-binding protein reagent interacts with a miRNA-binding protein associated with miRNA to form immunoprecipitated miRNA complexes; and releasing miRNA from the immunoprecipitated miRNA complexes. Examples of miRNA-binding proteins that can be associated with circulating miRNAs are further provided. |
