| 摘要 |
Provided are a cationic lipid which facilitates the introduction of a nucleic acid into, for example, a cell or the like; a composition containing the cationic lipid and a nucleic acid; a method for introducing a nucleic acid into a cell by using a composition containing the cationic lipid and the nucleic acid; and the like. The cationic lipid is, for example, a cationic lipid represented by formula (A): formula (A):;;(wherein R1 is alkenyl or the like, R2 is alkenyl or the like, R3 and R4 are each alkyl, or are combined together to form alkylene, or R3 and R5 are combined together to form alkylene, R5 is a hydrogen atom or the like, or is combined together with R3 to form alkylene, X1 is alkylene, and X2 is a single bond or alkylene). |
| 主权项 |
1. A cationic lipid represented by formula (A):(wherein
R1 is linear or branched alkyl, alkenyl, or alkynyl, each having 8 to 24 carbon atoms, R2 is linear or branched alkyl, alkenyl, or alkynyl, each having 8 to 24 carbon atoms, alkoxyethyl, alkoxypropyl, alkenyloxyethyl, alkenyloxypropyl, alkynyloxyethyl, or alkynyloxypropyl, R3 and R4 may be the same or different and are each alkyl having 1 to 3 carbon atoms, or are combined together to form alkylene having 2 to 8 carbon atoms, or R3 and R5 are combined together to form alkylene having 2 to 8 carbon atoms, R5 is a hydrogen atom, alkyl having 1 to 6 carbon atoms, alkenyl having 3 to 6 carbon atoms, amino, monoalkylamino, hydroxy, alkoxy, carbamoyl, monoalkylcarbamoyl, dialkylcarbamoyl, or alkyl having 1 to 6 carbon atoms or alkenyl having 3 to 6 carbon atoms, each substituted with one to three of the same or different substituents selected from amino, monoalkylamino, hydroxy, alkoxy, carbamoyl, monoalkylcarbamoyl, and dialkylcarbamoyl, or is combined together with R3 to form alkylene having 2 to 8 carbon atoms, X1 is alkylene having 1 to 6 carbon atoms, and X2 is a single bond or alkylene having 1 to 6 carbon atoms, provided that the sum of the number of carbon atoms in X1 and X2 is 7 or less, and when R5 is a hydrogen atom, X2 is a single bond, and when R5 and R3 are combined together to form alkylene having 2 to 6 carbon atoms, X2 is a single bond, or methylene or ethylene), formula (B):(wherein
R6 is linear or branched alkyl, alkenyl, or alkynyl, each having 8 to 24 carbon atoms, and R7 is linear or branched alkyl, alkenyl, or alkynyl, each having 8 to 24 carbon atoms, alkoxyethyl, alkoxypropyl, alkenyloxyethyl, alkenyloxypropyl, alkynyloxyethyl, or alkynyloxypropyl), or formula (C):(wherein
R8 is linear or branched alkyl, alkenyl, or alkynyl, each having 8 to 24 carbon atoms, R9 is linear or branched alkyl, alkenyl, or alkynyl, each having 8 to 24 carbon atoms, alkoxyethyl, alkoxypropyl, alkenyloxyethyl, alkenyloxypropyl, alkynyloxyethyl, or alkynyloxypropyl, X3 is alkylene having 1 to 3 carbon atoms, and R10 is a hydrogen atom or alkyl having 1 to 3 carbon atoms). |
