Methods and Compositions for Determining Smoking Status

摘要

Peripheral blood markers are provided whose expression levels correlate with smoking status. Predictive models developed using highly informative markers are disclosed, along with systems, kits, and methods for using the markers to provide a biochemical surrogate for a subject's smoking status. In some embodiments, the smoking-related disease is chronic obstructive pulmonary disease, chronic bronchitis, emphysema, lung cancer, and/or asthma.

主权项

1. A computer-implemented method for scoring a sample obtained from a subject, wherein said score indicates said subject's smoking status, comprising:
obtaining a dataset associated with said sample, wherein said dataset comprises data representing quantitative expression level values for two or more of marker 1, marker 2, marker 3, marker 4, and marker 5, wherein marker 1 is CLDND1 or IL7R, wherein marker 2 is LRRN3 or CCR7, wherein marker 3 is MUC1 or FOXP3, wherein marker 4 is GOPC or MCM3, and wherein marker 5 is LEF1 or CCR7; and determining, by a computer processor, a score via mathematical combination of the data from said dataset, wherein said score is indicative of said subject's smoking status.