发明名称 |
PRODUCTION CELL LINE ENHANCERS |
摘要 |
The present invention relates to discovery of the ectopic expression of EDEM2 in a production cell to improve the yield of a useful multi-subunit protein. Thus, the present invention provides for production cell lines, such as the canonical mammalian biopharmaceutical production cell—the CHO cell, containing recombinant polynucleotides encoding EDEM2. Also disclosed is a production cell containing both an EDEM2-encoding polynucleotide as well an XBP1-encoding polynucleotide. Improved titers of antibodies produced by these cell lines are disclosed, as well as the improved cell densities attained by these cells in culture. |
申请公布号 |
US2015175688(A1) |
申请公布日期 |
2015.06.25 |
申请号 |
US201414555220 |
申请日期 |
2014.11.26 |
申请人 |
REGENERON PHARMACEUTICALS, INC. |
发明人 |
CHEN Gang;BURAKOV Darya;DESHPANDE Dipali |
分类号 |
C07K16/22;C12N15/85 |
主分类号 |
C07K16/22 |
代理机构 |
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代理人 |
|
主权项 |
1. An isolated polynucleotide comprising a nucleotide sequence, which encodes an EDEM2, a multi-subunit protein, and operably linked to a promoter, wherein the EDEM2 comprises an amino acid sequence that is at least 92% identical to SEQ ID NO:1. |
地址 |
TARRYTOWN NY US |