| 摘要 |
The present invention relates to the field of plant breeding and disease resistance in respect of lignin degradation of plant by Macrophomina phaseolina (“M. phaseolina”). The present invention discloses isolated polynucleotide encoding of the lignin degrading enzymes, produced by Macrophomina phaseolina, comprising and consisting of nucleotide sequence set forth in SEQ. ID Nos. 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, 62, 65, 68, 71, 74, 77, 80, 83, 86, 89, 92, 95, 98, 101, 104, 107, 110, 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 76, 79, 82, 85, 88, 91, 94, 97, 100, 103, 106 and 109 or the complement of such sequences. The present invention also relates to isolated polypeptide encoded by the polynucleotide sequences set forth in SEQ ID Nos. 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 63, 66, 69, 72, 75, 78, 81, 84, 87, 90, 93, 96, 99, 102, 105, 108 and 111; a recombinant gene construct comprising the polynucleotide; a transformant and a transgenic fungus comprising the recombinant gene construct, having enhanced production of lignin degrading enzyme. The invention provides polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomerase to fermentable sugar in saccharification process of biomass. |
