Methods for detecting antibodies

摘要

The present invention relates to methods of detecting Anti-Drug Antibodies. The present invention also relates to methods of monitoring patients undergoing therapeutic antibody treatment. The invention further relates to kits suitable for the implementation of the above methods.

主权项

1. A method for immunodetecting an Anti-Drug Antibody (ADA) in a sample from a patient treated with a drug, wherein the drug is a therapeutic antibody or etanercept, said therapeutic antibody or etanercept being directed against a target antigen, the method comprising:
a) verifying the presence of the target antigen in the sample; b) if present, treating the sample with acid to neutralize the target antigen in the sample, capture of said target antigen by an affinity reagent or immuno-neutralization of said target antigen; and c) immuno-detecting the presence or amount of said ADA in said sample.