Nanonozzle device arrays: their preparation and use for macromolecular analysis

摘要

Constricted nanochannel devices suitable for use in analysis of macromolecular structure, including DNA sequencing, are disclosed. Also disclosed are methods for fabricating such devices and for analyzing macromolecules using such devices.

主权项

1. A device for analyzing a polynucleotide macromolecule, comprising:
two or more fluid reservoirs joined by a fluid pathway; a nanochannel having an effective inner diameter between about 10 nm and about 500 nm; a discrete constriction in or at a terminal end of the nanochannel, having an effective inner diameter that is no more than about 40% of the effective inner diameter of the nanochannel, wherein the nanochannel and the constriction are located in the fluid pathway between the reservoirs, wherein the constriction is capable of maintaining a linearized polynucleotide macromolecule passing across the constriction in a linearized form, wherein the constriction is configured to locally reduce the effective inner diameter of the nanochannel to be about 0.5 nm to about 100 nm; and a sensor associated with the device located to detect a signal from at least a portion of the polynucleotide macromolecule as it passes through the constriction.