Loci associated charcoal rot drought complex tolerance in soybean

摘要

The invention relates to methods and compositions for identifying soybean plants that are tolerant, have improved tolerance or are susceptible to Charcoal Rot Drought Complex. The methods use molecular genetic markers to identify, select and/or construct tolerant plants or identify and counter-select susceptible plants. Soybean plants that display tolerance or improved tolerance to Charcoal Rot Drought Complex that are generated by the methods of the invention are also a feature of the invention.

主权项

1. A method of selecting a first soybean plant or germplasm that displays tolerance or improved tolerance to Charcoal Rot Drought Complex (CRDC), the method comprising:
(a) isolating nucleic acids from a genome of the first soybean plant or germplasm; (b) detecting in the first soybean plant or germplasm at least one allele of a quantitative trait locus that is associated with the tolerance or improved tolerance, wherein said quantitative trait locus is localized to a chromosomal interval selected from the group consisting of (i) a chromosomal interval flanked by and including markers Satt467 and Satt416 on linkage group B2, and (ii) a chromosomal interval flanked by and including markers A605—1 and A519—2 on linkage group D1b wherein the at least one allele is detected using a composition comprising a detectable label; and, (c) selecting said first soybean plant or germplasm, or selecting a progeny of said first soybean plant or germplasm, comprising at least one allele associated with tolerance or improved tolerance to CRDC.