Fusion proteins of collagen-binding domain and parathyroid hormone

摘要

Fusion proteins containing active agonist or antagonist fragments of parathyroid hormone (PTH) and parathyroid hormone related peptide (PTHrP) coupled to a collagen-binding domain are presented. The fusion proteins can be used to promote bone growth, to promote hair growth, to prevent cancer metastasis to bone, to promote immune reconstitution with a bone marrow stem cell transplant, to promote mobilization of bone marrow stem cells for collection for autologous stem cell transplant, and to treat renal osteodystrophy. Pharmaceutical agents comprising a collagen-binding polypeptide segment linked to a non-peptidyl PTH/PTHrP receptor agonist or antagonist are also presented.

主权项

1. A method of promoting bone growth, promoting hair growth, promoting tissue growth, promoting immune reconstitution, promoting bone marrow stem cell mobilization and treating myocardial infarction comprising administering to a mammal an effective amount of a composition comprising a ColH or ColG bacterial collagen-binding polypeptide segment linked to a PTH/PTHrP receptor agonist,
wherein the collagen-binding polypeptide segment comprises a polypeptide with at least 90% identity to residues 38-158 of SEQ ID NO: 1; wherein the PTH/PTHrP receptor agonist comprises residues 1-14 of SEQ ID NO: 1, and wherein the composition promotes bone growth, promotes hair growth, promotes tissue growth, promotes immune reconstitution, promotes bone marrow stem cell mobilization or treats myocardial infarction.