ErbB-2 receptor targeting peptide

摘要

The ErbB-2 receptor, a member of the tyrosine kinase type 1 family of receptors, has been implicated in many human malignancies. Bacteriophage display technology was employed to identify peptides that bound to the extracellular domain of human ErbB-2. The peptide KCCYSL, most frequently occurring in the affinity selected population, was chemically synthesized and characterized for its binding activities to the recombinant extracellular domain of ErbB-2. The synthetic peptide exhibits high specificity to ErbB-2 as well as to ErbB-1, another member of ErbB family. Thus, this peptide can be employed in cancer imaging or therapeutic agent targeting to malignant cells ErbB-2 receptor.

主权项

1. A method for diagnosing ErbB-2-positive cancer in a subject comprising:
(a) administering to said subject a peptide-diagnostic agent complex, wherein said peptide is between 6 and about 100 residues in length and comprises the sequence KCCYSL (SEQ ID NO: 1) and wherein the peptide binds to ErbB-2; and (b) assessing the amount and/or localization in said subject, of the diagnostic agent.