发明名称 |
Láminas de células para la formación de la ectocórnea, método para producir las mismas y método para usar las mismas |
摘要 |
<p>The present invention has as its objective providing acorneal epithelium forming cell sheet that will adhere well to an anterior segment tissue. To attain this objective, a corneal epithelium forming cell sheet is produced by a process comprising the steps of cultivating under specified conditions corneal epithelium forming cells on a cell culture support comprising a substrate having its surface covered with a temperature responsive polymer of which the hydrating force varies in a temperature range of 0-80 DEG C, optionally stratifying the layer of cultured cells, and thereafter, (1) adjusting the temperature of the culture solution to either above an upper critical dissolution temperature or below a lower critical dissolution temperature, (2) bringing the cultured corneal epithelium forming cells into close contact with a carrier, and (3) detaching the sheet together with the carrier under specified conditions.</p> |
申请公布号 |
ES2538696(T3) |
申请公布日期 |
2015.06.23 |
申请号 |
ES20040708896T |
申请日期 |
2004.02.06 |
申请人 |
CELLSEED INC.;NISHIDA, KOHJI |
发明人 |
OKANO, TERUO;NISHIDA, KOHJI;YAMATO, MASAYUKI |
分类号 |
A61L27/38;C12N11/02;A61F2/14;A61F9/007;A61L27/00;C12N5/00;C12N5/07;C12N5/071 |
主分类号 |
A61L27/38 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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