发明名称 Substrate used for cell migration assays and method for cell migration assays
摘要 This invention provides a means for cell migration assays by regulating the cells adhered to a substrate so that they migrate to a given region, which is a substrate used for cell culture comprising: a base material comprising a conductive region and an insulating region provided thereon and cell-adhesive regions and non-cell-adhesive regions provided in the conductive region and the insulating region, respectively, wherein a cell-adhesive region is positioned adjacent to a non-cell-adhesive region in the conductive region, and a cell-adhesive region is positioned adjacent to a non-cell-adhesive region in the insulating region.
申请公布号 US9062341(B2) 申请公布日期 2015.06.23
申请号 US201013878726 申请日期 2010.12.06
申请人 DAI NIPPON PRINTING CO., LTD. 发明人 Yokoyama Naoki;Akai Tomonori;Takasugi Yuya
分类号 C12Q1/04 主分类号 C12Q1/04
代理机构 Lucas & Mercanti, LLP 代理人 Lucas & Mercanti, LLP
主权项 1. A cell culture substrate, comprising a base material comprising a conductive region and an insulating region provided thereon, cell-adhesive regions and non-cell-adhesive regions provided in the conductive region, and non-cell-adhesive regions provided in the insulating region, wherein one of the cell-adhesive regions is positioned adjacent to one of the non-cell-adhesive regions in the conductive region, the conductive region comprises a line-shaped conductive region, the insulating region is disposed on both sides of the line-shaped conductive region along a longitudinal direction of the line-shaped conductive region, the line-shaped conductive region comprises one or more of the cell-adhesive regions, wherein the one or more of the cell-adhesive regions are entirely or partially composed of the line-shaped conductive region(s) and are provided at one position or at two or more positions different along the longitudinal direction, when the line-shaped conductive region is a part of the one or more of the cell-adhesive regions, the one or more of the cell-adhesive regions further include the insulating region adjacent to the line-shaped conductive region, and the line-shaped conductive region and the insulating region outside the one or more of the cell-adhesive regions are the non-cell-adhesive regions, and when a dimension of one of the cell-adhesive regions in the longitudinal direction of the line-shaped conductive region is defined as a length of the one of the cell-adhesive regions and a dimension of the one of the cell-adhesive regions in a direction orthogonal to the longitudinal direction is defined as a width of the one of the cell-adhesive regions, the length and the width of the one of the cell-adhesive regions are 1 μm to 500 μm, and a line width of the line-shaped conductive region outside the one of the cell-adhesive regions is 0.1 μm to 10 μm, and the line width is smaller than the width of the one of the cell-adhesive regions adjacent thereto.
地址 Tokyo JP