Slow magic angle spinning nuclear magnetic resonance device and process for metabolomics profiling of tissues and biofluids

摘要

A slow Magic-Angle Spinning NMR device and method are detailed that provide high resolution and high sensitivity metabolic profiling of biological samples. A new 1H-PASS sequence suppresses line broadening in the various biological samples. The device and method allow metabolic changes in small animals to be tracked through continuous investigations of minimally-invasive blood and tissue biopsy samples over a sustained period and allow intact biological objects with sizes up to a few centimeters to be studied.

主权项

1. A slow Magic Angle Spinning (MAS) Nuclear Magnetic Resonance (NMR) device, comprising:
a sample spinning probe configured to rotate a sample inclined at a magic angle with respect to the B0 magnetic field at a slow spinning rate, the probe includes a rotor with a sample tube adapter configured to hold a sample tube of varied dimensions containing a sample of a variable size, quantity, and/or volume in the rotor when introduced into a support body of the probe; and a switchable LC resonator comprising a first static RF coil coupled to one or more capacitors that defines an RF circuit, the LC resonator attaches to a switchable plug component that inserts into the support body of the probe, the LC resonator when inserted in the support body inductively couples the first static RF coil with a second static RF coil disposed therein that enhances the sample filling factor between the sample and the first RF coil and the sensitivity of sample analyses therein.