Method for producing pyrroloquinoline quinone using a bacterium of the genus <i>Methylobacterium </i>or <i>Hyphomicrobium</i>

摘要

The present invention provides a method for producing PQQ using a bacterium belonging to the genera Methylobacterium or Hyphomicrobium, which has been modified to enhance the expression of pqq gene cluster and/or gene(s) encoding a precursor for PQQ biosynthesis.

主权项

1. A method for producing pyrroloquinoline quinone (PQQ) comprising:
A) cultivating in a culture medium a bacterium belonging to the genus Hyphomicrobium, and B) collecting PQQ from the culture medium, wherein the bacterium has been modified to enhance expression of a pqqABCDE gene cluster from Hyphomicrobium dentirificans by a method selected from the group consisting of: (i)increasing the copy number of the gene cluster, (ii) introducing multiple copies of the gene cluster into the chromosome of said bacterium, (iii) placing the gene cluster under the control of a potent promoter, and (iv) combinations thereof.