Enzyme directed oil biosynthesis in microalgae

摘要

The present invention is related to biosynthetic oil compositions and methods of making thereof. In some embodiments, the invention relates to the use of endogenous enzymes in plants capable of synthesizing oil. In preferred embodiments, said plants are algae. In further embodiments, said algae are from the family Chlamydomonas, Nannochloropsis, Dunaliella, Chiarella and Scenedesmus. In still further embodiments, said endogenous enzymes are diacylglycerol acyltransferases.

主权项

1. A method comprising:
a) providing:
i) algae, andii) a vector comprising a nucleic acid encoding a diacylglycerol acyltransferase operably linked to a promoter, wherein said nucleic acid has greater than 90% sequence identity to a sequence is selected from the group consisting of SEQ ID NO: 5, SEQ ID NO: 8 and SEQ ID NO: 11; b) transfecting said algae with said vector under conditions such that said diacylglycerol acyltransferase is expressed.