发明名称 METHODS FOR PRODUCING TRANSFORMED CELLS
摘要 PROBLEM TO BE SOLVED: To provide an easy and time-saving method for producing a transformed cell capable of intracellular expression of an RNA of interest, without previously linking the promoter sequence and the DNA sequence for the RNA expression.SOLUTION: A transformed cell capable of intracellularly expressing an RNA of interest is prepared by introducing into a cell a linear double-stranded DNA having a promoter sequence, and a linear double-stranded DNA comprising a DNA sequence for RNA expression and a terminator sequence in this order. By using such a production method, a transformed cell that expresses RNA of interest can be produced easily and in a short period of time.
申请公布号 JP2015112086(A) 申请公布日期 2015.06.22
申请号 JP20130258265 申请日期 2013.12.13
申请人 YAMAGUCHI UNIV 发明人 NAKAMURA MIKIKO;AKATA TOMOHARU;HOSHIDA SHOJI
分类号 C12N5/10;C12N15/00;C12N15/09 主分类号 C12N5/10
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