METHODS OF IMPROVING MULTIPLE PROTEIN PROPERTIES

摘要

FIELD: medicine, pharmaceutics.SUBSTANCE: claimed invention relates to method of supplying versions of protease or amylase with efficiency value in, at least one test of interest, exceeding index of efficiency for said enzyme of wild type. Multiple enzyme versions, encompassing certain range of the total surface charge or electrokinetic potential in, at least, one test of interest, are tested. Such test includes measurement of: efficiency of washing, substrate binding, enzyme inhibition; levels of expression, stability in detergent and/or thermal activity. Value of surface charge or electrokinetic potential, at which efficiency in test constitutes at least 50% of the maximal one, is identified. New set (library) of enzyme variants, enriched with members, possessing values of at least 50% of the maximal value, in said, at least, one test of interest within the limits of said charge or potential value. Said set of enzyme variants and, at least, one enzyme of wild type are tested in said, at least, one test of interest. If value of efficiency index is higher than index for wild type enzyme, enzyme variants, demonstrating improved result, are identified.EFFECT: application of invention provides novel method of obtaining variants of enzymes with improved activity.19 cl, 32 dwg, 14 tbl, 15 ex