METHOD OF SEPARATING LACTOFERRIN OF HUMAN AND GOAT BY MEANS OF DIFFERENTIAL IMMUNOAFFINITY CHROMATOGRAPHY USING SINGLE-DOMAIN MINI-ANTIBODIES

摘要

FIELD: biotechnology.SUBSTANCE: method comprises immunoaffinity chromatography using mini-antibodies a-hLF-1 and a-hLF-4, which amino acid sequences are presented as SEQ ID NO:1 and SEQ ID NO:2. The invention may be used to obtain highly purified fraction of human lactoferrin.EFFECT: invention enables to carry out with high efficiency the separation of proteins of lactoferrin of human and goat consisting in milk, using the single-domain mini-antibodies.6 dwg, 2 ex