METHODS AND APPARATUS FOR PERFORMING MASS SPECTROMETRY

摘要

The present disclosure relates, in part, to MS apparatus, methods, and/or software, having improved selectivity, sensitivity, specificity, resolution, mass accuracy and dynamic range over conventional MS technologies. In particular, the technology relates to apparatus, methods, and/or software wherein a combination of in-source fragmentation, ion mobility separation, and/or time-aligned parallel (TAP) sample ion fragmentations are utilized in mass spectrometry for the analysis of samples.

主权项

1. A method of forming structural information of a sample using mass spectrometry, comprising:
optimizing ionization source conditions to produce first generation product ions at the source having substantially maximum intensity; mass selecting, from the first generation product ions, ions having a mass of interest; fragmenting at least a portion of the mass-selected first generation product ions to produce second generation product ions; separating at least a portion of the second generation product ions by mobility; fragmenting a portion of the mobility separated second generation product ions to produce third generation product ions, and maintaining a remaining portion of the mobility separated second generation ions; and detecting, by a detector, the third generation product ions and the remaining portion of the mobility separated second generation product ions to form a series of mass spectra, each mass spectrum being associated with a different mobility value of the second generation product ions.