HYDROGEL-BASED VASCULAR LINEAGE CELL GROWTH MEDIA AND USES THEREOF

摘要

A medium for growing vascular lineage cells is described. The vascular lineage cell growth medium includes an oligosaccharide-based hydrogel and a growth factor that promotes vascularization by vascular lineage cells.

主权项

1. A method of preparing a vascular lineage cell growth medium comprising
chemically bonding an adhesion promoter to an oligosaccharide, combining the chemically bonded adhesion promoter and oligosaccharide with at least one growth factor, adding a crosslinking peptide moiety, curing the combination to form a hydrogel, wherein the hydrogel has a Young's modulus between about 10 Pa and about 500 Pa; and wherein the growth factor is an amount sufficient to promote vasculogenesis and tube formation, and sufficient to stimulate MMP production in vascular lineage cells.