APPARATUS AND METHODS FOR PREPARATIVE LIQUID CHROMATOGRAPHY

摘要

A method of liquid chromatography includes providing one or more solvent reservoirs, providing a solvent pump, drawing one or more solvents into the pump in response to a pressure drop that promotes outgassing of the solvents, and dispersing outgassed bubbles into smaller bubbles to promote re-dissolution of the gas. A liquid-chromatography apparatus includes at least two solvent reservoirs, a pump, at least one bubble-dispersing unit that receives a pressurized flow of proportioned solvents from the pump, and a control unit. The control unit includes a processor and a memory that stores instructions; the control unit controls proportioning of solvents to obtain a preselected solvent composition, and pumping at flow rates to support preparative-scale or process-scale liquid chromatography.

主权项

1. A method of liquid chromatography, comprising:
providing at least one solvent reservoir containing at least one solvent having dissolved gas; providing a pump comprising at least one piston disposed in a chamber in fluid communication with the at least one solvent reservoir; drawing the at least one solvent into the pump in response to a pressure drop that promotes formation of bubbles due to outgassing of the at least one solvent; and dispersing the bubbles into smaller bubbles, after the bubbles exit the pump, to promote re-dissolution of the gas.