FERMENTATION PROCESS

摘要

The present invention relates to improved processes for culturing bacteria, in particular to processes for perfusion suspension culturing of bacteria in a fermenter, wherein the culture medium including the bacteria is circulated over a separation system in alternating tangential flow, wherein the separation system removes a filtrate containing inhibitory metabolites from the culture medium.

主权项

1. A process for culturing a bacterial strain in a fermenter comprising the steps of:
a) adding a liquid growth medium to a fermenter; b) seeding the growth medium with bacteria to form a culture medium; and c) growing the bacteria in perfusion suspension culture, wherein the culture medium including the bacteria is circulated over a separation system in alternating tangential flow, and the separation system removes a filtrate containing spent medium from the culture medium and retains the bacteria in the culture medium.