AMYLASE-DEFICIENT STRAIN

摘要

The present invention relates to a mutant microbial host cell which has been modified, preferably in its genome, to result in a deficiency in the production of a polypeptide having α-amylase activity AmyC or an homologous thereof. It has been surprisingly found that when the mutant microbial host cell according to the invention is used in a method to produce a compound of interest, for example an enzyme, an improved purity of the product is obtained.

主权项

1. A mutant microbial host cell which has been modified, optionally in genome thereof, to result in a deficiency in production of a polypeptide having α-amylase activity selected from the group consisting of:
a. a polypeptide according to SEQ ID NO: 3 or 7 or a polypeptide at least 70% identical thereto; b. a mature polypeptide comprised in SEQ ID NO: 3 or 7 or a polypeptide at least 70% identical thereto; c. a polypeptide encoded by a polynucleotide according to SEQ ID NO: 1, 2, 5 or 6 or encoded by a polynucleotide at least 70% identical to SEQ ID NO: 1, 2, 5 or 6; d. a polypeptide encoded by a polynucleotide capable of hybridising to a polynucleotide according to SEQ ID NO: 1, 2, 5 or 6 or capable of hybridising to the complementary strand of a polynucleotide according to SEQ ID NO: 1, 2, 5 or 6; if compared with a parent microbial host cell which has not been modified and measured under the same conditions.