| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for obtaining an optimized humanized form of immunoglobulin which is not substantially immunogenic to a human, according to a technique based on structural data derived from crystallographic study and experimentally obtained.SOLUTION: The present invention provides a humanized anti-NGF antibody to NGF, or a fragment thereof which comprises (a) a VH region having a first CDR, a second CDR comprising a humanized amino acid sequence, and a third CDR; and (b) a VL region having the first CDR, the second CDR, and the third CDR; and is obtained by a method comprising steps of (i) obtaining crystal structures of the VH and VL region of anti-NGF antibody αD11, (ii) performing preliminary selection of a framework receptor of a human origin or humanized antibody, (iii) identifying the VH and VL region of the human origin of the anti-NGF antibody αD11, (iv) inserting a sequence of the CDR region of the anti-NGF antibody αD11 into a human sequence, (v) causing reverse mutation of one or more amino acid residues of the human VH and VL region. |
