METHODS FOR TARGETED TRANSGENE-INTEGRATION USING CUSTOM SITE-SPECIFIC DNA RECOMBINASES

摘要

The invention relates to biotechnology and provides novel methods for sequence-specific or sequence-directed transcription activator-like effector recombinase-mediated integration of DNA sequences of interest into host genomes. The invention also provides methods of use for novel plant transformation vectors and expression cassettes, which include novel combinations of chimeric recombinases with plant expression and transformation elements. Methods for gene-targeting, DNA sequence removal, genome modification, and molecular breeding are also provided.

主权项

1. A method of integrating a nucleic acid sequence into a selected genomic locus comprising:
a) transforming a host cell with:
i) at least one donor DNA construct comprising a first TALER target sequence and an exogenous DNA sequence;ii) at least one nucleic acid sequence encoding a TALER, wherein the TALER forms part of a tetramer and mediates recombination between the first TALER target sequence and a second TALER target sequence located in the host cell genome; and b) identifying a transformed host cell comprising the donor DNA construct integrated at a selected genomic locus in said host cell.