MICROSCOPE AND METHOD FOR SPIM MICROSCOPY

摘要

A microscope, in particular according to any of the preceding claims, consisting of an illuminating device, comprising an illumination light source and an illumination beam path for illuminating the specimen with a light sheet, a detection device for detecting light emitted by the specimen, and an imaging optical unit, which images the specimen via an imaging objective in an imaging beam path at least partly onto the detection device, wherein the light sheet is essentially planar at the focus of the imaging objective or in a defined plane in proximity of the geometrical focus of the imaging objective, and wherein the imaging objective has an optical axis, which intersects the plane of the light sheet at an angle that is different from zero, preferably perpendicularly, wherein an amplitude and/or phase filter is provided in the illumination beam path, said filter acting as a sine spatial filter in that it limits the illumination light in at least one spatial direction by filtering the spatial frequencies that occur with a sine function and/or in that the illumination light is limited with regard to the phase and amplitude thereof in at least one spatial direction by a sine filter function, and/or a combined amplitude and phase filter is provided in the illumination beam path, said filter shaping the light sheet by influencing the transmission profile of the illumination light distribution with a sine filter function.

主权项

1. A microscope, comprising
an imaging optical unit, which absorbs light emitted by a specimen into a first aperture angle via an imaging objective in a first direction, a detection device for detecting the fight emitted by the specimen into the first aperture angle, said light being absorbed by means of the imaging optical unit, and an illuminating device, having an illumination light source with a wavelength λ and an illumination beam path for illuminating the specimen with an illumination light distribution in a second direction, wherein the second direction is perpendicular to the first direction,said illuminating device having means such that said illumination light distribution is constant within a section in the second direction,
the power of the illumination light being less than 20% within a cone put up by the first aperture angle outside an area of an expansion in the first direction, wherein the following shall apply:
LY<2λ,LZ>8λ.