NON-VIRAL EPISOMAL SUICIDE CONSTRUCT

摘要

The invention provides compositions and methods for the selective expression of a target gene in a subset of cells. In one embodiment, the present invention provides a construct comprising a first nucleic acid sequence comprising an episomal maintenance element and a second nucleic acid sequence comprising a target gene wherein the expression of the episomal maintenance element is regulated by a constitutive promoter and the expression of the target gene is regulated, by a non-constitutive promoter. The construct is able to maintain episomal state no matter whether the target gene is expressed in the cell.