Improved methods for fermentative production of docosahexaenoic acid

摘要

The invention comprises a process of producing polyunsaturated fatty acids from Thraustochytriales at high availability of oxygen by submerged fermentation in a batch, semi-continuous or continuous mode. Batch fermentation of more than 200g/L and up to 450 g/L of dextrose added right in the beginning of fermentation was achieved with productivity of DHA achieved for higher than 10g/Liter. The process comprises maintaining at least 20% to 30% dissolved oxygen and sometimes up to around 50% or more.