发明名称 |
A METHOD FOR MONITORING PARP ACTIVITY IN CELLS BY PARP ACTIVATION |
摘要 |
The current invention provides for improved methods for determining PAR, and uses thereof. These improved methods involve an incubation step at a low temperature, for example around, but above, the freezing point after a PARP activation step. This improves the assay's sensitivity in comparison to current assay's performance. For example, sensitivity can be improved in methods assaying cells obtained from subjects such as human patients. The current invention also allows to provide methods to screen compounds for modulating PARG and/or PARP activity. |
申请公布号 |
EP2882864(A1) |
申请公布日期 |
2015.06.17 |
申请号 |
EP20130756723 |
申请日期 |
2013.08.08 |
申请人 |
STICHTING HET NEDERLANDS KANKER INSTITUUT |
发明人 |
VENS, CONCHITA;PLUIM, DERK;VERHEIJ, MARCEL;SCHELLENS, JOHANNES HENRICUS MATTHIAS |
分类号 |
C12Q1/48 |
主分类号 |
C12Q1/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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