发明名称 |
DETECTION OF ABL MUTANT BY ALLELE-SPECIFIC AMPLIFICATON |
摘要 |
A method of selectively producing and amplifying a cDNA sequence of a target allele of a gene, wherein the target allele is a mutant allele or is a specific allele of a polymorphic gene, the method comprising: (a) providing a sample comprising an mRNA transcript of the target allele; (b) performing a reverse-transcription reaction to generate a cDNA sequence from the mRNA transcript, and (c) amplifying the cDNA of the target allele; wherein the reverse-transcription reaction is selective for reverse transcription of the mRNA transcript of the target allele over an mRNA transcript of an alternative allele of the same gene. |
申请公布号 |
EP2780478(A4) |
申请公布日期 |
2015.06.17 |
申请号 |
EP20120849514 |
申请日期 |
2012.11.16 |
申请人 |
CLARIENT DIAGNOSTIC SERVICES, INC. |
发明人 |
CHOPPA, PAUL, CHRISTOPHER |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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