| 摘要 |
The present invention provides compositions, methods, and systems for generating antigen-specific long-term memory T-cells using mTOR pathway inhibitors. The present invention provides compositions, systems, and methods for administering antigen-specific long-term memory T-cells to a subject (e.g., to a subject with cancer in adoptive transfer type of procedures). |
| 主权项 |
1. A method of generating a population of antigen-specific memory T-cells comprising:
a) processing an initial sample from a subject under conditions such that an isolated T-cell sample is generated, wherein said initial sample is selected from: a peripheral blood sample, a spleen sample, and a lymph node sample, and wherein said isolated T-cell sample comprises purified cells, wherein substantially all of said purified cells present in said T-cell sample are unprimed CD44− CD8+ T-cells as a result of said processing; and b) contacting said isolated T-cell sample, which contains said unprimed CD44− CD8− T-cells, with: i) an antigen, ii) antigen presenting cells, and iii) an mTOR pathway inhibiting agent, under conditions such that a memory T-cell sample is generated that comprises memory T-cells specific to said antigen, wherein substantially all of said memory T-cells present in said memory T-cell sample are CD44− CD62L+ CD8+; wherein said mTOR pathway inhibiting agent is selected from the group consisting of: rapamycin, temsirolimus, everolimus torin, and deforolimus. |
