Stem cell separating material and method of separation

摘要

The present invention has its object to provide a material for separating stem cell and a filter for separating stem cell, each is capable of selectively separating and recovering, in a simple and easy manner, stem cells from body fluids or biological tissue-derived treated fluids, a method for separating and recovering stem cells, and stem cells obtained by such method. The present invention is a material for separating stem cell which has a density K of 1.0×104≦K≦1.0×106 and a fiber diameter of 3 to 40 μm; a filter for separating stem cell which comprises the material for separating stem cell as packed in a container having a fluid inlet port and a fluid outlet port; a method of separating and recovering stem cells which comprises using the material for separating stem cell or the filter for separating stem cell; and a method of producing a multipotent cell fraction.

主权项

1. A method of separating and recovering stem cells from a body fluid or a biological tissue-derived treated fluid
which comprises introducing a body fluid or the biological tissue-derived treated fluid into a filter comprising a material for separating stem cell having a density K of 1.0×104≦K≦1.0×106 g/m3 and a fiber diameter of 3 to 40 μm, as packed in a container having a fluid inlet port and a fluid outlet port, through the fluid inlet port thereof, and a cell recovering fluid is caused to flow from the fluid outlet side to thereby recover adherent stem cells captured by the material for separating stem cell, and further culturing the adherent stem cells in a culture dish, a culture flask, or a culture bag, wherein the adherent stem cells are mesenchymal stem cells or bone marrow stromal cells, wherein the body fluid is bone marrow fluid, and wherein the biological tissue-derived treated fluid is a treated fluid obtained by disintegration of at least one biological tissue selected from the group consisting of subcutaneous fat, visceral fat, white fat, and brown fat.