Method for isolating a cyclohexapeptide

摘要

The present invention relates to a method for isolating acyclohexapeptide and to a novel crystalline form of caspofungin diacetate thus obtained.

主权项

1. A method for isolating crystalline caspofungin diacetate comprising the steps of:
(a) contacting a solution comprising caspofungin and water with a resin; (b) removing the liquid phase from the mixture obtained in step (a); (c) eluting the caspofungin by contacting material retained after removal of the liquid phase in step (b) by addition of an organic solvent to obtain a mixture thereof; (d) separating resin from the mixture obtained in step (c); (e) crystallizing the caspofungin from the solution retained after removal of the resin to obtain crystalline caspofungin diacetate in a mixture with the solution; and (f) isolating the crystalline caspofungin diacetate from the mixture obtained in step (e), wherein the crystalline caspofungin diacetate obtained according to step (e) is characterized by data selected from the group consisting of:
(i) an XRD powder diffraction pattern with peaks at 2.92±0.2, 5.06±0.2 and 5.88±0.2 degrees 2-theta,(ii) an XRD powder diffraction pattern as depicted in FIG. 1, or(iii) combinations of (i) and (ii), whereina ratio between an intensity of the peak at 2.92±0.2 degrees 2-theta and an intensity of any peak in the region 9.0±0.2 degrees 2-theta is at least 10.