| 摘要 |
The present invention relates to a cell culture apparatus and a mass automatic cell culture device having it. There is provided a cell culture apparatus comprising a cylindrical cell culture flask which is air-tightly sealed and formed of a transparent material to observe an internal portion thereof, an injection unit for supplying the cell and the culture solution into the cell culture flask, a collection unit for discharging the cells and the culture solution from the cell culture flask, and a cell culture flask receiving part which is comprised of first and second vertical frames in which a plurality of injecting parts and collecting parts, and plate type connection parts which are respectively disposed between upper ends and lower ends of the two opposed vertical frames to be spaced apart from each other in regular intervals, and a mass automatic cell culture device having it. |
| 主权项 |
1. A cell culture apparatus, comprising:
(a) a plurality of cylindrical cell culture flasks which are each: (i) air-tightly sealed to culture a cell using a culture solution and various gases, (ii) formed of a transparent material to permit observation of an internal portion thereof, (iii) formed into a cylindrical shape with a predetermined height, and (iv) comprise a culture space for cell culture; (b) a plurality of injection units for supplying the cell and the culture solution into the plurality of cell culture flasks; (c) a plurality of collection units for collecting the cell and the culture solution from the plurality of cell culture flasks; and (d) a cell culture flask receiving part which is comprised of (i) first and second vertical frames in which a plurality of injecting parts and a plurality of collecting parts for respectively receiving the plurality of injection units and the plurality of collection units are vertically formed to be opposed to each other, and (ii) plate-shaped connection parts for receiving the plurality of cell culture flasks, which are respectively disposed between upper ends and lower ends of the first and second vertical frames spaced apart from each other in regular intervals, thereby connecting the first and second vertical frames, wherein the plurality of injecting parts and the plurality of collecting parts are aligned along the first and second vertical frames, respectively, and individually provide a space for a reciprocating action of the plurality of injection units and the plurality of collection units, wherein the first vertical frame is connected with the second vertical frame through the plate-shaped connection parts, wherein at a side surface of each of the cell culture flasks is formed a culture solution inlet port for introducing the culture solution or the cell into the culture space, a culture solution outlet port for discharging the culture solution or the cell from the culture space, a gas inlet port and a gas outlet port to introduce and discharge the various gases into/from the culture space, and a foreign substance inlet port to introduce a foreign substance, wherein each of the culture solution inlet port and the culture solution outlet port protrude from an outer surface of each of the cell culture flasks and have a narrow end, wherein all ports are formed along a side surface of each of the cell culture flasks in order of the culture solution inlet port, the gas inlet port, the gas outlet port, the culture solution outlet port, and the foreign substance inlet port, wherein each of the cell culture flasks are directly connected to one of the plurality of the injection units and one of the plurality of the collection units through the culture solution inlet port and the culture solution outlet port; wherein the plurality of injection units and the plurality of collection units each comprise: (i) a syringe part with a power supply; (ii) a culture solution storing part which is connected with the syringe part, wherein the culture solution storing part is formed into a bottle shape for receiving the culture solution; (iii) a contact part which is connected with the culture solution storing part and is configured to flow the culture solution to each of the cell culture flasks, wherein the contact part is formed at an end of the syringe part and is funnel-shaped; (iv) a piston member which is disposed to be reciprocated inside the culture solution storing part; and (v) a connection member of which one end is connected with the piston member and the other end is connected with the power supply, wherein the connection member comprises a linear rod or a lead screw. |
