Attenuated RNA virus and applications thereof

摘要

The invention encompasses an attenuated RNA virus and methods of using an attenuated RNA virus. The RNA virus comprises, in part, an ion channel protein comprising a peptide tag.

主权项

1. An isolated influenza virus, wherein the virus comprises a M2 protein comprising a peptide tag at the carboxyl terminus, wherein
(a) the in vitro function of the M2 protein comprising the peptide tag is not altered compared to the in vitro function of the native M2 protein, (b) replication of the virus in vitro is similar to that of a virulent strain of the same virus, and the replication of the virus in a host is reduced relative to that of a virulent strain of the same virus, and (c) the virulence of the virus in a host is reduced relative to that of a virulent strain of the same virus.